Mosaic analysis of stem cell function and wound healing in the mouse corneal epithelium.

ABSTRACT:

Background

The mouse corneal epithelium is a continuously renewing 5-6 cell thick protective layer covering the corneal surface, which regenerates rapidly when injured. It is maintained by peripherally located limbal stem cells (LSCs) that produce transient amplifying cells (TACs) which proliferate, migrate centripetally, differentiate and are eventually shed from the epithelial surface. LSC activity is required both for normal tissue maintenance and wound healing. Mosaic analysis can provide insights into LSC function, cell movement and cell mixing during tissue maintenance and repair. The present study investigates cell streaming during corneal maintenance and repair and changes in LSC function with age.

Results

The initial pattern of corneal epithelial patches in XLacZ+/-

SUBMITTER: Mort RL 

PROVIDER: S-EPMC2639382 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature