Coherent anti-Stokes Raman scattering imaging of lipids in cancer metastasis.

ABSTRACT:

Background

Lipid-rich tumours have been associated with increased cancer metastasis and aggressive clinical behaviours. Nonetheless, pathologists cannot classify lipid-rich tumours as a clinically distinctive form of carcinoma due to a lack of mechanistic understanding on the roles of lipids in cancer development.

Methods

Coherent anti-Stokes Raman scattering (CARS) microscopy is employed to study cancer cell behaviours in excess lipid environments in vivo and in vitro. The impacts of a high fat diet on cancer development are evaluated in a Balb/c mice cancer model. Intravital flow cytometry and histology are employed to enumerate cancer cell escape to the bloodstream and metastasis to lung tissues, respectively. Cancer cell motility and tissue invasion capability are also e

SUBMITTER: Le TT 

PROVIDER: S-EPMC2640413 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature