Combinatorial effects of environmental parameters on transcriptional regulation in Saccharomyces cerevisiae: a quantitative analysis of a compendium of chemostat-based transcriptome data.

ABSTRACT:

Background

Microorganisms adapt their transcriptome by integrating multiple chemical and physical signals from their environment. Shake-flask cultivation does not allow precise manipulation of individual culture parameters and therefore precludes a quantitative analysis of the (combinatorial) influence of these parameters on transcriptional regulation. Steady-state chemostat cultures, which do enable accurate control, measurement and manipulation of individual cultivation parameters (e.g. specific growth rate, temperature, identity of the growth-limiting nutrient) appear to provide a promising experimental platform for such a combinatorial analysis.

Results

A microarray compendium of 170 steady-state chemostat cultures of the yeast Saccharomyces cerevisiae is presented and a

SUBMITTER: Knijnenburg TA 

PROVIDER: S-EPMC2640415 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature