Characterization of a Plasmodium falciparum macrophage-migration inhibitory factor homologue.

ABSTRACT:

Background

Macrophage-migration inhibitory factor (MIF), one of the first cytokines described, has a broad range of proinflammatory properties. The genome sequencing project of Plasmodium falciparum identified a parasite homologue of MIF. The protein is expressed during the asexual blood stages of the parasite life cycle that cause malarial disease. The identification of a parasite homologue of MIF raised the question of whether it affects monocyte function in a manner similar to its human counterpart.

Methods

Recombinant P. falciparum MIF (PfMIF) was generated and used in vitro to assess its influence on monocyte function. Antibodies generated against PfMIF were used to determine the expression profile and localization of the protein in blood-stage parasites. Antibody respo

SUBMITTER: Cordery DV 

PROVIDER: S-EPMC2640454 | biostudies-literature | 2007 Mar

REPOSITORIES: biostudies-literature