Vesicular stomatitis virus matrix protein mutations that affect association with host membranes and viral nucleocapsids.

ABSTRACT: Viral matrix (M) proteins bind the nucleoprotein core (nucleocapsid) to host membranes during the process of virus assembly by budding. Previous studies using truncated M proteins had implicated the N-terminal 50 amino acids of the vesicular stomatitis virus M protein in binding both membranes and nucleocapsids and a sequence from amino acids 75-106 as an additional membrane binding region. Structure-based mutations were introduced into these two regions, and their effects on membrane association and incorporation into nucleocapsid-M protein complexes were determined using quantitative assays. The results confirmed that the N terminus of M protein is involved in association with plasma membranes as well as nucleocapsids, although these two activities were differentially affected by individ

SUBMITTER: Dancho B 

PROVIDER: S-EPMC2640965 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature