Temporal profiling of the adipocyte proteome during differentiation using a five-plex SILAC based strategy.

ABSTRACT: The adipose tissue has important secretory and endocrine functions in humans. The regulation of adipocyte differentiation has been actively pursued using transcriptomic methods over the last several years. Quantitative proteomics has emerged as a promising approach to obtain temporal profiles of biological processes such as differentiation. Stable isotope labeling with amino acids in cell culture (SILAC) is a simple and robust method for labeling proteins in vivo. Here, we describe the development and application of a five-plex SILAC experiment using four different heavy stable isotopic forms of arginine to study the nuclear proteome and the secretome during the course of adipocyte differentiation. Tandem mass spectrometry analysis using a quadrupole time-of-flight instrument resulted in i

SUBMITTER: Molina H 

PROVIDER: S-EPMC2642533 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature