NOEnet--use of NOE networks for NMR resonance assignment of proteins with known 3D structure.

ABSTRACT:

Motivation

A prerequisite for any protein study by NMR is the assignment of the resonances from the (15)N-(1)H HSQC spectrum to their corresponding atoms of the protein backbone. Usually, this assignment is obtained by analyzing triple resonance NMR experiments. An alternative assignment strategy exploits the information given by an already available 3D structure of the same or a homologous protein. Up to now, the algorithms that have been developed around the structure-based assignment strategy have the important drawbacks that they cannot guarantee a high assignment accuracy near to 100%.

Results

We propose here a new program, called NOEnet, implementing an efficient complete search algorithm that ensures the correctness of the assignment results. NOEnet exploits the netwo

SUBMITTER: Stratmann D 

PROVIDER: S-EPMC2642640 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature