A compact phage display human scFv library for selection of antibodies to a wide variety of antigens.

ABSTRACT:

Background

Phage display technology is a powerful new tool for making antibodies outside the immune system, thus avoiding the use of experimental animals. In the early days, it was postulated that this technique would eventually replace hybridoma technology and animal immunisations. However, since this technology emerged more than 20 years ago, there have only been a handful reports on the construction and application of phage display antibody libraries world-wide.

Results

Here we report the simplest and highly efficient method for the construction of a highly useful human single chain variable fragment (scFv) library. The least number of oligonucleotide primers, electroporations and ligation reactions were used to generate a library of 1.5 x 108 individual clones, without g

SUBMITTER: Pansri P 

PROVIDER: S-EPMC2642811 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature