Differential expression of Kir4.1 and aquaporin 4 in the retina from endotoxin-induced uveitis rat.

ABSTRACT:

Purpose

The inwardly rectifying potassium channel protein Kir4.1 and the water channel protein aquaporin-4 (AQP4) have been suggested to play essential roles in the potassium and water homeostasis of the retina. In this study, we investigated the expression of Kir4.1 and AQP4 in the retina during endotoxin-induced uveitis (EIU) in rats.

Methods

EIU was induced in male Wistar rats by intravitreal injection of lipopolysaccharide (LPS). The severity of the EIU was evaluated by clinical and histopathological examination. The expression of Kir4.1 and AQP4 in the retina was detected by semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR), Western blotting, and immunohistochemical staining.

Results

In the animal model of EIU, the clinical changes correla

SUBMITTER: Liu XQ 

PROVIDER: S-EPMC2642914 | biostudies-literature | 2007 Mar

REPOSITORIES: biostudies-literature