Elevated insulin signaling disrupts the growth and differentiation pattern of the mouse lens.

ABSTRACT:

Purpose

Insulin and insulin-like growth factors (IGFs) are putative regulators of cell proliferation and differentiation during lens development. Transgenic mice that overexpress IGF-1 in the lens have been previously described. To further understand the ocular functions of this growth factor family, the in vivo effects of insulin expression on lens development were investigated using transgenic mice.

Methods

Expression of insulin receptor (IR) and IGF-1 receptor (IGF-1R) in mouse lens was examined by reverse-transcriptase-polymerase chain reaction (RT-PCR) and in situ hybridization. Transgenic mice that overexpress insulin in the lens were generated using two different promoters: a fiber-cell specific alphaA-crystallin (alphaA) promoter and a modified alphaA-promoter linked

SUBMITTER: Xie L 

PROVIDER: S-EPMC2642934 | biostudies-literature | 2007 Mar

REPOSITORIES: biostudies-literature