Ontology highlight
ABSTRACT:
SUBMITTER: McBride A
PROVIDER: S-EPMC2642990 | biostudies-literature | 2009 Jan
REPOSITORIES: biostudies-literature
McBride Andrew A Ghilagaber Stephanos S Nikolaev Andrei A Hardie D Grahame DG
Cell metabolism 20090101 1
AMPK beta subunits contain a conserved domain that causes association with glycogen. Although glycogen availability is known to affect AMPK regulation in vivo, the molecular mechanism for this has not been clear. We now show that AMPK is inhibited by glycogen, particularly preparations with high branching content. We synthesized a series of branched oligosaccharides and show that those with a single alpha1-->6 branch are allosteric inhibitors that also inhibit phosphorylation by upstream kinases ...[more]