Mutation detection by real-time PCR: a simple, robust and highly selective method.

ABSTRACT:

Background

Molecular tests for diagnosis of disease, particularly cancer, are gaining increased acceptance by physicians and their patients for disease prognosis and selection of treatment options. Gene expression profiles and genetic mutations are key parameters used for the molecular characterization of tumors. A variety of methods exist for mutation analysis but the development of assays with high selectivity tends to require a process of trial and error, and few are compatible with real-time PCR. We sought to develop a real-time PCR-based mutation assay methodology that successfully addresses these issues.

Methodology/principal findings

The method we describe is based on the widely used TaqMan real-time PCR technology, and combines Allele-Specific PCR with a Blocking rea

SUBMITTER: Morlan J 

PROVIDER: S-EPMC2642996 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature