Cigarette smoke induces cellular senescence via Werner's syndrome protein down-regulation.

ABSTRACT:

Rationale

Werner's syndrome is a genetic disorder that causes premature aging due to loss-of-function mutations in a gene encoding a member of the RecQ helicase family. Both Werner's syndrome and cigarette smoking accelerate aging. No studies have examined the effect of cigarette smoke on Werner's syndrome protein.

Objectives

To investigate the role of Werner's syndrome protein in cigarette smoke-induced cellular senescence.

Methods

Cellular senescence and amounts of Werner's syndrome protein were measured in fibroblasts isolated from patients with emphysema and compared with age-matched nonsmokers. The in vitro effects of cigarette smoke on amounts of Werner's syndrome protein, function, and senescence were also evaluated in primary human lung fibroblasts and epithel

SUBMITTER: Nyunoya T 

PROVIDER: S-EPMC2643077 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature