Characterization of two novel alpha-glucosidases from Bifidobacterium breve UCC2003.

ABSTRACT: Two alpha-glucosidase-encoding genes (agl1 and agl2) from Bifidobacterium breve UCC2003 were identified and characterized. Based on their similarity to characterized carbohydrate hydrolases, the Agl1 and Agl2 enzymes are both assigned to a subgroup of the glycosyl hydrolase family 13, the alpha-1,6-glucosidases (EC 3.2.1.10). Recombinant Agl1 and Agl2 into which a His(12) sequence was incorporated (Agl1(His) and Agl2(His), respectively) exhibited hydrolytic activity towards panose, isomaltose, isomaltotriose, and four sucrose isomers--palatinose, trehalulose, turanose, and maltulose--while also degrading trehalose and, to a lesser extent, nigerose. The preferred substrates for both enzymes were panose, isomaltose, and trehalulose. Furthermore, the pH and temperature optima for both enzymes

SUBMITTER: Pokusaeva K 

PROVIDER: S-EPMC2643558 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature