Design and validation of a microarray for detection, hemagglutinin subtyping, and pathotyping of avian influenza viruses.

ABSTRACT: Continuing threats of devastating outbreaks in poultry and of human infections caused by highly pathogenic avian influenza virus (HPAIV) H5N1 emphasize the need for the further development of rapid and reliable methods of virus detection and characterization. Here we report on the design and comprehensive validation of a low-density microarray as a diagnostic tool for the detection and typing of avian influenza virus (AIV). The array consists of one probe for the conserved matrix gene and 97 probes targeting the HA(0) cleavage-site region. Following fragment amplification by a generic PCR approach, the array enables AIV detection, hemagglutinin (HA) subtyping, and pathotyping within a single assay. For validation, a panel of 92 influenza A viruses which included 43 reference strains repres

SUBMITTER: Gall A 

PROVIDER: S-EPMC2643657 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature