Analysis of human papillomavirus type 18 load and integration status from low-grade cervical lesion to invasive cervical cancer.

ABSTRACT: The clinical value of viral load and integration testing for human papillomavirus (HPV) remains unclear. Data on HPV type 18 (HPV18) is limited. We examined the HPV18 viral load and integration status of 78 women with normal cervix or neoplasia. While the crude viral load appeared to increase with lesion severity, the association was not significant after normalization with sample cellularity. Unlike reports for HPV16, the amino-terminal 1 region of HPV18 E2 was most frequently (71.0%) disrupted, representing the best marker for integration. A substantial proportion (57.1%) of invasive cancers harbored only the episomal genome, thus jeopardizing the clinical value of integration testing. A large proportion (41.7%) of normal/low-grade lesions showed viral integration, suggesting that integr

SUBMITTER: Cheung JL 

PROVIDER: S-EPMC2643667 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature