Postrecruitment regulation of RNA polymerase II directs rapid signaling responses at the promoters of estrogen target genes.

ABSTRACT: Under classical models for signal-dependent transcription in eukaryotes, DNA-binding activator proteins regulate the recruitment of RNA polymerase II (Pol II) to a set of target promoters. However, recent studies, as well as our results herein, show that Pol II is widely distributed (i.e., "preloaded") at the promoters of many genes prior to specific signaling events. How Pol II recruitment and Pol II preloading fit within a unified model of gene regulation is unclear. In addition, the mechanisms through which cellular signals activate preloaded Pol II across mammalian genomes remain largely unknown. We show here that the predominant genomic outcome of estrogen signaling is the postrecruitment regulation of Pol II activity at target gene promoters, likely through specific changes in Pol II

SUBMITTER: Kininis M 

PROVIDER: S-EPMC2643830 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature