Membrane transporters and protein traffic networks differentially affecting metal tolerance: a genomic phenotyping study in yeast.

ABSTRACT:

Background

The cellular mechanisms that underlie metal toxicity and detoxification are rather variegated and incompletely understood. Genomic phenotyping was used to assess the roles played by all nonessential Saccharomyces cerevisiae proteins in modulating cell viability after exposure to cadmium, nickel, and other metals.

Results

A number of novel genes and pathways that affect multimetal as well as metal-specific tolerance were discovered. Although the vacuole emerged as a major hot spot for metal detoxification, we also identified a number of pathways that play a more general, less direct role in promoting cell survival under stress conditions (for example, mRNA decay, nucleocytoplasmic transport, and iron acquisition) as well as proteins that are more proximally related

SUBMITTER: Ruotolo R 

PROVIDER: S-EPMC2643938 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature