Perceiving molecular evolution processes in Escherichia coli by comprehensive metabolite and gene expression profiling.

ABSTRACT:

Background

Evolutionary changes that are due to different environmental conditions can be examined based on the various molecular aspects that constitute a cell, namely transcript, protein, or metabolite abundance. We analyzed changes in transcript and metabolite abundance in evolved and ancestor strains in three different evolutionary conditions - excess nutrient adaptation, prolonged stationary phase adaptation, and adaptation because of environmental shift - in two different strains of bacterium Escherichia coli K-12 (MG1655 and DH10B).

Results

Metabolite profiling of 84 identified metabolites revealed that most of the metabolites involved in the tricarboxylic acid cycle and nucleotide metabolism were altered in both of the excess nutrient evolved lines. Gene expression p

SUBMITTER: Vijayendran C 

PROVIDER: S-EPMC2643943 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature