High-resolution mapping of heterochromatin redistribution in a Drosophila position-effect variegation model.

ABSTRACT:

Background

Position-effect variegation (PEV) is the stochastic transcriptional silencing of a gene positioned adjacent to heterochromatin. white-mottled X-chromosomal inversions in Drosophila are classic PEV models that show variegation of the eye color gene white due to its relocation next to pericentric heterochromatin. It has been suggested that in these models the spreading of heterochromatin across the rearrangement breakpoint causes the silencing of white. However, the extent of this spreading and the precise pattern of heterochromatin redistribution have remained unclear. To obtain insight into the mechanism of PEV, we constructed high-resolution binding maps of Heterochromatin Protein 1 (HP1) on white-mottled chromosomes.

Results

We find that HP1 invades euchromatin

SUBMITTER: Vogel MJ 

PROVIDER: S-EPMC2644302 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature