Identification of genes differentially expressed between benign and osteopontin transformed rat mammary epithelial cells.

ABSTRACT:

Background

Osteopontin is a secreted, integrin-binding and phosphorylated acidic glycoprotein which has an important role in tumor progression.

Findings

In this study, we have utilized suppressive subtractive hybridization (SSH) to evaluate OPN regulated gene expression, using the Rama 37 benign non-invasive rat mammary cell line and a subclone, Rama 37-OPN. Rama 37-OPN was produced by stably transfecting Rama 37 with an OPN expression vector and it demonstrates increased malignant properties in vitro. Sequence and expression array analysis of the respective cDNA libraries of over 1600 subtracted cDNA fragments revealed 982 ESTs, 45 novel sequences and 659 known genes. The known up-regulated genes in the Rama 37-OPN library code for proteins with a variety of functions inclu

SUBMITTER: Kurisetty VV 

PROVIDER: S-EPMC2644310 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature