Gene expression profile analysis of human hepatocellular carcinoma using SAGE and LongSAGE.

ABSTRACT:

Background

Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide and the second cancer killer in China. The initiation and malignant transformation of cancer result from accumulation of genetic changes in the sequences or expression level of cancer-related genes. It is of particular importance to determine gene expression profiles of cancers on a global scale. SAGE and LongSAGE have been developed for this purpose.

Methods

We performed SAGE in normal liver and HCC samples as well as the liver cancer cell line HepG2. Meanwhile, the same HCC sample was simultaneously analyzed using LongSAGE. Computational analysis was carried out to identify differentially expressed genes between normal liver and HCC which were further validated by real-time quantitative R

SUBMITTER: Dong H 

PROVIDER: S-EPMC2644313 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature