Defining developmental potency and cell lineage trajectories by expression profiling of differentiating mouse embryonic stem cells.

ABSTRACT: Biologists rely on morphology, function and specific markers to define the differentiation status of cells. Transcript profiling has expanded the repertoire of these markers by providing the snapshot of cellular status that reflects the activity of all genes. However, such data have been used only to assess relative similarities and differences of these cells. Here we show that principal component analysis of global gene expression profiles map cells in multidimensional transcript profile space and the positions of differentiating cells progress in a stepwise manner along trajectories starting from undifferentiated embryonic stem (ES) cells located in the apex. We present three 'cell lineage trajectories', which represent the differentiation of ES cells into the first three lineages in mam

SUBMITTER: Aiba K 

PROVIDER: S-EPMC2644347 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature