Phase I trial of interleukin-12 plasmid electroporation in patients with metastatic melanoma.

ABSTRACT:

Purpose

Gene-based immunotherapy for cancer is limited by the lack of safe, efficient, reproducible, and titratable delivery methods. Direct injection of DNA into tissue, although safer than viral vectors, suffers from low gene transfer efficiency. In vivo electroporation, in preclinical models, significantly enhances gene transfer efficiency while retaining the safety advantages of plasmid DNA.

Patients and methods

A phase I dose escalation trial of plasmid interleukin (IL)-12 electroporation was carried out in patients with metastatic melanoma. Patients received electroporation on days 1, 5, and 8 during a single 39-day cycle, into metastatic melanoma lesions with six 100-mus pulses at a 1,300-V/cm electric field through a penetrating six-electrode array immediately after

SUBMITTER: Daud AI 

PROVIDER: S-EPMC2645111 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature