Microarray reveals complement components are regulated in the serum-deprived rat retinal ganglion cell line.

ABSTRACT:

Purpose

Glaucoma is a progressive eye disease that leads to blindness due to loss of retinal ganglion cells (RGCs). There are difficulties in using primary cultures of purified RGC to study this pathophysiology. RGC-5, a transformed not RGC line, expresses several markers characteristic of the RGCs. The aim of this study was to generate a genome-wide gene expression of RGC-5 following serum deprivation and to identify candidate genes that may be involved in the signal transduction pathways.

Methods

Apoptosis in the transformed rat RGC-5 was induced by serum deprivation for 0, 8, 24, 48, and 96 h. Briefly, 400 ng of RNA from each sample was reverse transcribed and labeled with Cy3 dye. Fragmented fluorescent cRNA was mixed with hybridization buffer and incubated at 60 degrees

SUBMITTER: Khalyfa A 

PROVIDER: S-EPMC2645904 | biostudies-literature | 2007 Feb

REPOSITORIES: biostudies-literature