New strategy for quantifying biological zinc by a modified zinpyr fluorescence sensor.

ABSTRACT: Substitution of one pyridine by pyrazine in each DPA appendage of ZP1 leads to a new zinc sensor, ZPP1, with a modified background fluorescence and zinc affinity. ZPP1 exhibits a two-step zinc response during fluorescence titrations, which leads to a new method for zinc quantification. The ability of ZPP1 to image and quantify zinc was demonstrated in pancreatic Min6 cells.

SUBMITTER: Zhang XA 

PROVIDER: S-EPMC2645955 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature