Reduced adipose tissue oxygenation in human obesity: evidence for rarefaction, macrophage chemotaxis, and inflammation without an angiogenic response.

ABSTRACT:

Objective

Based on rodent studies, we examined the hypothesis that increased adipose tissue (AT) mass in obesity without an adequate support of vascularization might lead to hypoxia, macrophage infiltration, and inflammation.

Research design and methods

Oxygen partial pressure (AT pO2) and AT temperature in abdominal AT (9 lean and 12 overweight/obese men and women) was measured by direct insertion of a polarographic Clark electrode. Body composition was measured by dual-energy X-ray absorptiometry, and insulin sensitivity was measured by hyperinsulinemic-euglycemic clamp. Abdominal subcutaneous tissue was used for staining, quantitative RT-PCR, and chemokine secretion assay.

Results

AT pO2 was lower in overweight/obese subjects than lean subjects (47 +/- 10.6 vs. 55

SUBMITTER: Pasarica M 

PROVIDER: S-EPMC2646071 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature