Absence of GAPDH regulation in tumor-cells of different origin under hypoxic conditions in - vitro.

ABSTRACT:

Background

Gene expression studies related to cancer diagnosis and treatment are important. In order to conduct such experiment accurately, absolutely reliable housekeeping genes are essential to normalize cancer related gene expression. The most important characteristics of such genes are their presence in all cells and their expression levels remain relatively constant under different experimental conditions. However, no single gene of this group of genes manifests always stable expression levels under all experimental conditions. Incorrect choice of housekeeping genes leads to interpretation errors of experimental results including evaluation and quantification of pathological gene expression. Here, we examined (a) the degree of GAPDH expression regulation in Hep-1-6 mouse hepat

SUBMITTER: Said HM 

PROVIDER: S-EPMC2646737 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature