Sensitive fluorescence detection of nucleic acids based on isothermal circular strand-displacement polymerization reaction.

ABSTRACT: Here we have developed a sensitive DNA amplified detection method based on isothermal strand-displacement polymerization reaction. This method takes advantage of both the hybridization property of DNA and the strand-displacement property of polymerase. Importantly, we demonstrate that our method produces a circular polymerization reaction activated by the target, which essentially allows it to self-detect. Functionally, this DNA system consists of a hairpin fluorescence probe, a short primer and polymerase. Upon recognition and hybridization with the target ssDNA, the stem of the hairpin probe is opened, after which the opened probe anneals with the primer and triggers the polymerization reaction. During this process of the polymerization reaction, a complementary DNA is synthesized and th

SUBMITTER: Guo Q 

PROVIDER: S-EPMC2647313 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature