Effects of magnesium and related divalent metal ions in topoisomerase structure and function.

ABSTRACT: The catalytic steps through which DNA topoisomerases produce their biological effects and the interference of drug molecules with the enzyme-DNA cleavage complex have been thoroughly investigated both from the biophysical and the biochemical point of view. This provides the basic structural insight on how this family of essential enzymes works in living systems and how their functions can be impaired by natural and synthetic compounds. Besides other factors, the physiological environment is known to affect substantially the biological properties of topoisomerases, a key role being played by metal ion cofactors, especially divalent ions (Mg(2+)), that are crucial to bestow and modulate catalytic activity by exploiting distinctive chemical features such as ionic size, hardness and characteri

SUBMITTER: Sissi C 

PROVIDER: S-EPMC2647314 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature