A Candida albicans early stage biofilm detachment event in rich medium.

ABSTRACT:

Background

Dispersal from Candida albicans biofilms that colonize catheters is implicated as a primary factor in the link between contaminated catheters and life threatening blood stream infections (BSI). Appropriate in vitro C. albicans biofilm models are needed to probe factors that induce detachment events.

Results

Using a flow through system to culture C. albicans biofilms we characterized a detachment process which culminates in dissociation of an entire early stage biofilm from a silicone elastomer surface. We analyzed the transcriptome response at time points that bracketed an abrupt transition in which a strong adhesive association with the surface is weakened in the initial stages of the process, and also compared batch and biofilm cultures at relevant time points.

SUBMITTER: Sellam A 

PROVIDER: S-EPMC2647545 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature