An Ac/Ds-mediated gene trap system for functional genomics in barley.

ABSTRACT:

Background

Gene trapping is a powerful tool for gene discovery and functional genomics in both animals and plants. Upon insertion of the gene trap construct into an expressed gene, splice donor and acceptor sites facilitate the generation of transcriptional fusions between the flanking sequence and the reporter. Consequently, detection of reporter gene expression allows the identification of genes based on their expression pattern. Up to now rice is the only cereal crop for which gene trap approaches exist. In this study we describe a gene trap system in barley (Hordeum vulgare L.) based on the maize transposable elements Ac/Ds.

Results

We generated gene trap barley lines by crossing Ac transposase expressing plants with multiple independent transformants carrying the Ds bas

SUBMITTER: Lazarow K 

PROVIDER: S-EPMC2647555 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature