Human initiator caspases trigger apoptotic and autophagic phenotypes in Saccharomyces cerevisiae.

ABSTRACT: Caspases are a family of proteases that participate in the progression and execution of the apoptotic program. However, regulation of the caspase activation and their substrates has not yet been fully elucidated. Here we explore the effect of the ectopic expression of the human initiator caspases-8 and -10 in Saccharomyces cerevisiae. Our results showed that the expression of human CASP10 and CASP8 triggers certain apoptotic markers such as a massive production of reactive oxygen species (ROS), chromatin condensation and phosphatidylserine externalization, finally leading to cell death. In response to hydroxyurea (HU), yeast cells expressing caspase-10 did not reduce the replication of DNA and escaped to the intra-S checkpoint of the cell cycle. In addition, caspase-10 expression induced y

SUBMITTER: Lisa-Santamaria P 

PROVIDER: S-EPMC2647587 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature