Growth phase- and cell division-dependent activation and inactivation of the {sigma}32 regulon in Escherichia coli.

ABSTRACT: Alternative sigma factors allow bacteria to reprogram global transcription rapidly and to adapt to changes in the environment. Here we report on growth- and cell division-dependent sigma(32) regulon activity in Escherichia coli in batch culture. By analyzing sigma(32) expression in growing cells, an increase in sigma(32) protein levels is observed during the first round of cell division after exit from stationary phase. Increased sigma(32) protein levels result from transcriptional activation of the rpoH gene. After the first round of bulk cell division, rpoH transcript levels and sigma(32) protein levels decrease again. The late-logarithmic phase and the transition to stationary phase are accompanied by a second increase in sigma(32) levels and enhanced stability of sigma(32) protein but

SUBMITTER: Wagner MA 

PROVIDER: S-EPMC2648200 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature