Development and validation of a gene expression oligo microarray for the gilthead sea bream (Sparus aurata).

ABSTRACT:

Background

Aquaculture represents the most sustainable alternative of seafood supply to substitute for the declining marine fisheries, but severe production bottlenecks remain to be solved. The application of genomic technologies offers much promise to rapidly increase our knowledge on biological processes in farmed species and overcome such bottlenecks. Here we present an integrated platform for mRNA expression profiling in the gilthead sea bream (Sparus aurata), a marine teleost of great importance for aquaculture.

Results

A public data base was constructed, consisting of 19,734 unique clusters (3,563 contigs and 16,171 singletons). Functional annotation was obtained for 8,021 clusters. Over 4,000 sequences were also associated with a GO entry. Two 60mer probes were design

SUBMITTER: Ferraresso S 

PROVIDER: S-EPMC2648989 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature