RDX induces aberrant expression of microRNAs in mouse brain and liver.

ABSTRACT:

Background

Although microRNAs (miRNAs) have been found to play an important role in many biological and metabolic processes, their functions in animal response to environmental toxicant exposure are largely unknown.

Objectives

We used hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX), a common environmental contaminant, as a toxicant stressor to investigate toxicant-induced changes in miRNA expression in B6C3F1 mice and the potential mechanism of RDX-induced toxic action.

Methods

B6C3F1 mice were fed diets with or without 5 mg/kg RDX for 28 days. After the feeding trials, we isolated RNAs from both brain and liver tissues and analyzed the expression profiles of 567 known mouse miRNAs using microarray and quantitative real-time polymerase chain reaction technologies.

Re

SUBMITTER: Zhang B 

PROVIDER: S-EPMC2649225 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature