CD133+ adult human retinal cells remain undifferentiated in Leukaemia Inhibitory Factor (LIF).

ABSTRACT:

Background

CD133 is a cell surface marker of haematopoietic stem and progenitor cells. Leukaemia inhibitory factor (LIF), sustains proliferation and not differentiation of embryonic stem cells. We used CD133 to purify adult human retinal cells and aimed to determine what effect LIF had on these cultures and whether they still had the ability to generate neurospheres.

Methods

Retinal cell suspensions were derived from adult human post-mortem tissue with ethical approval. With magnetic automated cell sorting (MACS) CD133+ retinal cells were enriched from post mortem adult human retina. CD133+ retinal cell phenotype was analysed by flow cytometry and cultured cells were observed for proliferative capacity, neuropshere generation and differentiation with or without LIF supplemen

SUBMITTER: Carter DA 

PROVIDER: S-EPMC2649894 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature