Focus on RNA isolation: obtaining RNA for microRNA (miRNA) expression profiling analyses of neural tissue.

ABSTRACT: MicroRNAs (miRNAs) are present in all known plant and animal tissues and appear to be somewhat concentrated in the mammalian nervous system. Many different miRNA expression profiling platforms have been described. However, relatively little research has been published to establish the importance of 'upstream' variables in RNA isolation for neural miRNA expression profiling. We tested whether apparent changes in miRNA expression profiles may be associated with tissue processing, RNA isolation techniques, or different cell types in the sample. RNA isolation was performed on a single brain sample using eight different RNA isolation methods, and results were correlated using a conventional miRNA microarray and then cross-referenced to Northern blots. Differing results were seen between samples

SUBMITTER: Wang WX 

PROVIDER: S-EPMC2650220 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature