The number and transmission of [PSI] prion seeds (Propagons) in the yeast Saccharomyces cerevisiae.

ABSTRACT:

Background

Yeast (Saccharomyces cerevisiae) prions are efficiently propagated and the on-going generation and transmission of prion seeds (propagons) to daughter cells during cell division ensures a high degree of mitotic stability. The reversible inhibition of the molecular chaperone Hsp104p by guanidine hydrochloride (GdnHCl) results in cell division-dependent elimination of yeast prions due to a block in propagon generation and the subsequent dilution out of propagons by cell division.

Principal findings

Analysing the kinetics of the GdnHCl-induced elimination of the yeast [PSI+] prion has allowed us to develop novel statistical models that aid our understanding of prion propagation in yeast cells. Here we describe the application of a new stochastic model that allows us

SUBMITTER: Byrne LJ 

PROVIDER: S-EPMC2650407 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature