Detection of molecular markers of antiviral resistance in influenza A (H5N1) viruses using a pyrosequencing method.

ABSTRACT: Resistance of influenza viruses to antiviral drugs can emerge following medication or may result from natural variation. Two classes of anti-influenza virus drugs targeting either the M2 protein (amantadine and rimantadine) or neuraminidase (NA; oseltamivir and zanamivir) are currently licensed. These drugs are expected to be important in controlling the early stages of a potential pandemic. In the present study, we describe how a pyrosequencing method can be used to rapidly detect established molecular markers of resistance to M2 blockers and NA inhibitors in influenza A (H5N1) viruses. The residues L26, V27, A30, S31, and G34 in the M2 protein were targeted for pyrosequencing. The NA residues for pyrosequencing analysis included the established markers of drug resistance (H274 and N294),

SUBMITTER: Deyde VM 

PROVIDER: S-EPMC2650582 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature