Fine expression profiling of full-length transcripts using a size-unbiased cDNA library prepared with the vector-capping method.

ABSTRACT: Recently, we have developed a vector-capping method for constructing a full-length cDNA library. In the present study, we performed in-depth analysis of the vector-capped cDNA library prepared from a single type of cell. As a result of single-pass sequencing analysis of 24,000 clones randomly isolated from the unamplified library, we identified 19,951 full-length cDNA clones whose intactness was confirmed by the presence of an additional G at their 5' end. The full-length cDNA content was >95%. Mapping these sequences to the human genome, we identified 4,513 transcriptional units that include 36 antisense transcripts against known genes. Comparison of the frequencies of abundant clones showed that the expression profiles of different libraries, including the distribution of transcriptional

SUBMITTER: Oshikawa M 

PROVIDER: S-EPMC2650634 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature