Loss of full length CtBP1 expression enhances the invasive potential of human melanoma.

ABSTRACT:

Background

The C-terminal binding protein 1 (CtBP1) is a known co-repressor of gene transcription. We recently revealed that CtBP1 expression is lost in melanoma cells and melanoma inhibitory activity (MIA) expression is subsequently increased. The present study was performed to evaluate a more general role of CtBP1 in human melanoma and identify further CtBP1-regulated target genes.

Methods

Sequence analysis and expression profile of CtBP1 in melanoma cell lines were done by PCR. Boyden Chamber assays and co-immunoprecipitation were performed to investigate the functional role of CtBP1. Gene expression analysis and micro array data were used to define target genes.

Results

Interestingly, we detected an alternative splice product of CtBP1 with unknown function whose e

SUBMITTER: Winklmeier A 

PROVIDER: S-EPMC2650708 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature