A ChIP-chip approach reveals a novel role for transcription factor IRF1 in the DNA damage response.

ABSTRACT: IRF1 is a transcription factor that regulates key processes in the immune system and in tumour suppression. To gain further insight into IRF1's role in these processes, we searched for new target genes by performing chromatin immunoprecipitation coupled to a CpG island microarray (ChIP-chip). Using this approach we identified 202 new IRF1-binding sites with high confidence. Functional categorization of the target genes revealed a surprising cadre of new roles that can be linked to IRF1. One of the major functional categories was the DNA damage response pathway. In order to further validate our findings, we show that IRF1 can regulate the mRNA expression of a number of the DNA damage response genes in our list. In particular, we demonstrate that the mRNA and protein levels of the DNA repair

SUBMITTER: Frontini M 

PROVIDER: S-EPMC2651779 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature