Incorporating double copies of a chromatin insulator into lentiviral vectors results in less viral integrants.

ABSTRACT:

Background

Lentiviral vectors hold great promise as gene transfer vectors in gene therapeutic settings. However, problems related to the risk of insertional mutagenesis, transgene silencing and positional effects have stalled the use of such vectors in the clinic. Chromatin insulators are boundary elements that can prevent enhancer-promoter interactions, if placed between these elements, and protect transgene cassettes from silencing and positional effects. It has been suggested that insulators can improve the safety and performance of lentiviral vectors. Therefore insulators have been incorporated into lentiviral vectors in order to enhance their safety profile and improve transgene expression. Commonly such insulator vectors are produced at lower titers than control vectors thus

SUBMITTER: Nielsen TT 

PROVIDER: S-EPMC2651870 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature