Characterisation of porin genes from Mycobacterium fortuitum and their impact on growth.

ABSTRACT:

Background

Highly pathogenic mycobacteria like Mycobacterium tuberculosis are characterised by their slow growth and their ability to reside and multiply in the very hostile phagosomal environment and a correlation between the growth rate of mycobacteria and their pathogenicity has been hypothesised. Here, porin genes from M. fortuitum were cloned and characterised to address their impact on the growth rate of fast-growing and pathogenic mycobacteria.

Results

Two genes encoding porins orthologous to MspA from M. smegmatis, porM1 and porM2, were cloned from M. fortuitum strains, which were originally isolated from human patients. Both porin genes were at least partially able to complement the mutations of a M. smegmatis mutant strain lacking the genes mspA and mspC with respe

SUBMITTER: Sharbati S 

PROVIDER: S-EPMC2651896 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature