Characteristics of age-related changes in cultured human vocal fold fibroblasts.

ABSTRACT:

Objectives/hypothesis

Normal human vocal fold fibroblast (hVFF) primary cell lines are unavailable commercially and are very difficult to acquire, subsequently little is known about their characteristics. The purpose of this study was to compare the morphological and proliferation characteristics and gene expression of hVFFs from different aged donors.

Study design

In vitro.

Methods

We developed three normal hVFF primary cell lines from donors aged 21 (21T), 59 (59T) and 79 (79T) years. We characterized their morphological features, proliferative abilities, telomere lengths, and their functional gene expression by quantitative real-time PCR.

Results

The 21T line maintained a typical spindle shape until passage 14 whereas 59T and 79T changed morphology to wider,

SUBMITTER: Chen X 

PROVIDER: S-EPMC2652878 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature