Improving the functional expression of a Bacillus licheniformis laccase by random and site-directed mutagenesis.

ABSTRACT:

Background

Laccases have huge potential for biotechnological applications due to their broad substrate spectrum and wide range of reactions they are able to catalyze. These include, for example, the formation and degradation of dimers, oligomers, polymers, and ring cleavage as well as oxidation of aromatic compounds. Potential applications of laccases include detoxification of industrial effluents, decolorization of textile dyes and the synthesis of natural products by, for instance, dimerization of phenolic acids. We have recently published a report on the cloning and characterization of a CotA Bacillus licheniformis laccase, an enzyme that catalyzes dimerization of phenolic acids. However, the broad application of this laccase is limited by its low expression level of 26 mg l-1 t

SUBMITTER: Koschorreck K 

PROVIDER: S-EPMC2653023 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature