Determination of kinetic parameters of enzyme-catalyzed reaction a + B + C --> products with the minimum number of velocity measurements.

ABSTRACT: Rapid-equilibrium rate equations are derived for the five different mechanisms for the enzymatic catalysis of A + B + C --> products using a computer. These rate equations are used to determine the minimum number of velocities required to estimate the values of the kinetic parameters. The rate equation for the completely ordered mechanism involves four kinetic parameters, and the rate equation for the completely random mechanism involves eight kinetic parameters. Therefore, the four to eight kinetic parameters can be estimated by determining four to eight velocities and solving four to eight simultaneous equations. General recommendations are made as to the choices of triplets of substrate concentrations {[A], [B], [C]} to be used to determine the velocities. The effects of 5% errors in th

SUBMITTER: Alberty RA 

PROVIDER: S-EPMC2654092 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature