A rapid biosensor-based method for quantification of free and glucose-conjugated salicylic acid.

ABSTRACT:

Background

Salicylic acid (SA) is an important signalling molecule in plant defenses against biotrophic pathogens. It is also involved in several other processes such as heat production, flowering, and germination. SA exists in the plant as free SA and as an inert glucose conjugate (salicylic acid 2-O-beta-D-glucoside or SAG). Recently, Huang et al. developed a bacterial biosensor that responds to free SA but not SAG, designated as Acinetobacter sp. ADPWH_lux. In this paper we describe an improved methodology for Acinetobacter sp. ADPWH_lux-based free SA quantification, enabling high-throughput analysis, and present an approach for the quantification of SAG from crude plant extracts.

Results

On the basis of the original biosensor-based method, we optimized extraction and qua

SUBMITTER: Defraia CT 

PROVIDER: S-EPMC2654556 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature