Quantitative proteomics of the archaeon Methanococcus maripaludis validated by microarray analysis and real time PCR.

ABSTRACT: For the archaeon Methanococcus maripaludis, a fully sequenced and annotated model species of hydrogenotrophic methanogen, we report validation of quantitative protein level expression ratios on a proteome-wide basis. Using an approach based on quantitative multidimensional capillary HPLC and quadrupole ion trap mass spectrometry, coverage of gene expression approached that currently achievable with transcription microarrays. Comprehensive mass spectrometry-based proteomics and spotted cDNA arrays were used to compare global protein and mRNA levels in a wild-type (S2) and mutant strain (S40) of M. maripaludis. Using linear regression with 652 expression ratios generated by both the proteomic and microarray methods, a product moment correlation coefficient of 0.24 was observed. The correlati

SUBMITTER: Xia Q 

PROVIDER: S-EPMC2655211 | biostudies-literature | 2006 May

REPOSITORIES: biostudies-literature